{"id":1341,"date":"2019-05-09T11:42:14","date_gmt":"2019-05-09T09:42:14","guid":{"rendered":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/?p=1341"},"modified":"2020-10-28T14:52:47","modified_gmt":"2020-10-28T13:52:47","slug":"un-modele-moleculaire-simplifier-pour-etudier-la-complementarite-lactase-lactose","status":"publish","type":"post","link":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/?p=1341","title":{"rendered":"Un mod\u00e8le mol\u00e9culaire simplifi\u00e9 pour \u00e9tudier la compl\u00e9mentarit\u00e9 lactase-lactose"},"content":{"rendered":"

Par Philippe Cosentino<\/em><\/p>\n

La lactase (b\u00eata galactosidase) est un t\u00e9tram\u00e8re, ce qui rend complexe son \u00e9tude en classe.<\/p>\n

Mod\u00e8le original : 1JYN (PDB)<\/p>\n

Nous proposons un mod\u00e8le simplifi\u00e9, limit\u00e9 \u00e0 un seul monom\u00e8re, et d\u00e9barrass\u00e9 de toutes les mol\u00e9cules de solvant, les ions, l’eau (plusieurs centaines) pr\u00e9sentes initialement dans\u00a0 le fichier (H2<\/sub>O, DMS, Na+, Mg++, etc.).<\/p>\n

Certaines mol\u00e9cules d’eau et des ions Mg++ interviennent dans la catalyse, mais nous avons choisi d\u00e9lib\u00e9r\u00e9ment de ne laisser que le lactose et l’enzyme.<\/p>\n

Le mod\u00e8le initial comportait une mutation emp\u00eachant la catalyse (sans quoi il aurait \u00e9t\u00e9 impossible de cristalliser le complexe), il a fallu modifier le fichier pour remplacer un Gln par Glu et ainsi \u00ab\u00a0annuler\u00a0\u00bb la mutation (merci Paul Pillot).<\/p>\n

\"Capture<\/a>

Capture d’\u00e9cran de Libmol utilisant ce mod\u00e8le<\/p><\/div>\n

Il peut \u00eatre ouvert avec Rastop, Libmol ou tout autre logiciel de visualisation mol\u00e9culaire.<\/p>\n

T\u00e9l\u00e9charger le mod\u00e8le (\u00e0 d\u00e9zipper) :<\/p>\n

lactase-lactose-leger<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Par Philippe Cosentino La lactase (b\u00eata galactosidase) est un t\u00e9tram\u00e8re, ce qui rend complexe son \u00e9tude en classe. Mod\u00e8le original : 1JYN (PDB) Nous proposons un mod\u00e8le simplifi\u00e9, limit\u00e9 \u00e0 un seul monom\u00e8re, et d\u00e9barrass\u00e9 de toutes les mol\u00e9cules de solvant, les ions, l’eau (plusieurs centaines) pr\u00e9sentes initialement dans\u00a0 le fichier (H2O, DMS, Na+, Mg++, … <\/p>\n

Lire la suite<\/a><\/p>\n","protected":false},"author":74,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"footnotes":""},"categories":[18,8,77,357],"tags":[170,169,168,167],"class_list":["post-1341","post","type-post","status-publish","format-standard","hentry","category-devant-ecran","category-logiciels","category-modeles-moleculaires","category-premiere","tag-170","tag-lactase","tag-lactose","tag-libmol","nodate","item-wrap"],"rttpg_featured_image_url":null,"rttpg_author":{"display_name":"Philippe COSENTINO","author_link":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/?author=74"},"rttpg_comment":0,"rttpg_category":"Devant l'\u00e9cran<\/a> Logiciels<\/a> Mod\u00e8les mol\u00e9culaires<\/a> Premi\u00e8re<\/a>","rttpg_excerpt":"Par Philippe Cosentino La lactase (b\u00eata galactosidase) est un t\u00e9tram\u00e8re, ce qui rend complexe son \u00e9tude en classe. Mod\u00e8le original : 1JYN (PDB) Nous proposons un mod\u00e8le simplifi\u00e9, limit\u00e9 \u00e0 un seul monom\u00e8re, et d\u00e9barrass\u00e9 de toutes les mol\u00e9cules de solvant, les ions, l’eau (plusieurs centaines) pr\u00e9sentes initialement dans\u00a0 le fichier (H2O, DMS, Na+, Mg++,\u2026","post_mailing_queue_ids":[],"_links":{"self":[{"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=\/wp\/v2\/posts\/1341","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=\/wp\/v2\/users\/74"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1341"}],"version-history":[{"count":10,"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=\/wp\/v2\/posts\/1341\/revisions"}],"predecessor-version":[{"id":1523,"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=\/wp\/v2\/posts\/1341\/revisions\/1523"}],"wp:attachment":[{"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1341"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1341"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pedagogie.ac-nice.fr\/svt\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1341"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}